MICROPROPAGATION OF Bauhinia acuminata L

Seeds of Bauhinia acuminata L. were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Seeds were germinated on MS medium supplemented with GA3 (0.10, 1.0 and 2.0 mgL -1 ) and without GA3. Satisfactory germination was observed at media with 1.0 mgL -l GA3. Subsequent propagation from plantlet was performed on MS medium supplemented with various concentrations of BA and NAA or IBA. For Shoot proliferation, combinations of BA and NAA (0.20+0.10, 0.30+0.10, 0.4+0.1, 0.50+0.10 and 1.0+0.10 mgL -l ) were used. Reasonable shoot formation was observed at 0.50 mgL -l BA + 0.10 mgL -l NAA. For rooting IBA (0.20, 0.60 and 0.80mgL 1 ) and NAA (0.20, 0.60 and 0.80mgL -1 ) were used. The highest numbers of roots were observed at NAA 0.60 mgL -1 .